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. 2010 Sep 16;15(9):6436–6451. doi: 10.3390/molecules15096436

Figure 3.

Figure 3

Effects of chrysophanol on the activation of NF-κB p65 and caspase-1 in DSS-treated colon tissue. At the end of experiment, the colon tissues were cut out and homogenized. (A) The levels of NF-κB p65 were evaluated by Western blot analysis; (B) The relative expression level of NF-κB was measured using an image analyzer; (C) Colon were removed and embedded in paraffin, and then 5 μm sections were prepared and stained with anti-NF-κB; (D) The enzymatic activity of caspase-1 was evaluated via a caspase colorimetric assay. 1) Control group; 2) DSS alone-treated group; 3) chrysophanol (5 mg/kg) + DSS treated group; 4) sulfasalazine (150 mg/kg) + DSS treated group.Values were represented in the mean ± S.E.M. (n = 5) of triplicate determinations from triplicate separate experiments (# p < 0.05 vs. control, * p < 0.05 vs. DSS alone).