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. 2010 Aug 30;15(9):5959–5970. doi: 10.3390/molecules15095956

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© 2010 by the authors;

licensee MDPI, Basel, Switzerland. This article is an Open Access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

Pharmacological assays to determine the effect of tannin in the hydroalcoholic (HA) extract of D. alata barks on mouse phrenic nerve-diaphragm preparations stimulated indirectly. Note that both the extract containing tannin [HA(+)] and the extract free of tannin [HA(-)] significantly improved the muscle twitch tension and hence decreased neuromuscular blockade promoted by Bothrops jararacussu (Bjssu) venom alone. However, HA(+) showed more efficicacy than HA (-). Each point represents the mean ± S.E.M. of the number of experiments (n) showed in the legend. *p < 0.05 compared to venom.