Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Caenorhabditis elegans) |
N2 | Caenorhabditis Genetics Center | RRID:WB-STRAIN:N2_(ancestral) | wild-type (Bristol) |
| Strain, strain background (C. elegans) | CL2166 | CGC | RRID:WB-STRAIN:CL2166 |
dvIs19 [(pAF15)gst
-4p::GFP::NLS] III |
| Strain, strain background (C. elegans) | SJ4005 | CGC | RRID:WB-STRAIN:SJ4005 | zcIs4 [hsp-4::GFP] V |
| Strain, strain background (C. elegans) |
SJ4100 | CGC | RRID:WB-
STRAIN:SJ4100 |
zcIs13 [hsp-6::GFP] |
| Strain, strain background (C. elegans) | CL2070 | CGC | RRID:WB-STRAIN:CL2070 | dvIs70 [hsp-16.2p::GFP + rol-6(su1006)] |
| Strain, strain background (C. elegans) |
SJ17 | CGC | RRID:WB-S
TRAIN:SJ17 |
xbp-1(zc12) III; zcIs4 V |
| Strain, strain background (C. elegans) | QV225 | CGC | RRID:WB-
STRAIN:QV225 |
skn-1(zj15) IV |
| Strain, strain background (C. elegans) |
PS3551 | CGC | RRID:WB-STRAIN:PS3551 | hsf-1(sy441) I |
| Strain, strain background (C. elegans) | CF1038 | CGC | RRID:WB-STRAIN:CF1038 | daf-16(mu86) I |
| Strain, strain background (C. elegans) | CB369 | CGC | RRID:WB-STRAIN:CB369 | unc-51(e369) V |
| Strain, strain background (C. elegans) | RB1206 | CGC | RRID:WB-STRAIN:RB1206 | rsks-1(ok1255) III |
| Strain, strain background (C. elegans) | CB3388 | CGC | RRID:WB-STRAIN:CB3388 | ncl-1(e1865) III |
| Strain, strain background (C. elegans) | CL2006 | CGC | RRID:WB-STRAIN:CL2006 | dvIs2(pCL12(unc-54:hu-Aβ 1–42)+pRF4) |
| Strain, strain background (C. elegans) | NL5901 | CGC | RRID:WB-STRAIN:NL5901 | pkIs2386 [α-synuclein::YFP unc-119(+)] |
| Strain, strain background (C. elegans) | MAH93 | Other |
glp-1(ar202), unc-119(ed3), ItIs38[pAA1; pie-1/GFP::PH(PLCdelta1); unc-119 (+)] III; ItIs37[pAA64; pie-1/mCHERRY::his-58, unc-119 (+)]IV); gift from M. Hansen |
|
| Strain, strain background (C. elegans) | MAH686 | Other | hlh-30(tm1978) IV; gift from M. Hansen | |
| Strain, strain background (C. elegans) | CMH5 | DOI: 10.1371/ journal.pone.0159989 |
atfs-1(tm4525) V | |
| Strain, strain background (C. elegans) |
BC20306 | Baillie Genome GFP Project, Simon Fraser University |
RRID:WB:-STRAIN:BC20306 | cyp-34A9::GFP |
| Cell line (Homo sapiens) | HeLa | ATCC | Cat # CCL-2, RRID:CVCL_0030 |
|
| Cell line (Mus musculus) | NIH 3T3 | ATCC | Cat # CRL-1658, RRID:CVCL_0594 |
|
| Cell line (H. sapiens) | HEK293DAX | DOI: 10.1016/j.celrep.2013.03.024 | ||
| Antibody | anti-actin (mouse monoclonal) | MP Biomedicals | Cat # 08691001, RRID:AB_2335127 |
(1:500) |
| Antibody | anti-Hsp60 (mouse monoclonal) |
ThermoFisher | Cat # MA3-012, RRID:AB_2121466 |
(1:250) |
| Antibody | anti-Hsp70/72 (mouse monoclonal) | Enzo | Cat # ADI-SPA-810, RRID:AB_10616513 |
(1:1000) |
| Antibody | anti-GRP78 (BiP) (rabbit polyclonal) | Abcam | Cat # ab21685, RRID:AB_2119834 |
(1:1000, from1 mg/ml) |
| Antibody | IRDye 800CW (secondary) | Li-Cor | Cat # 926–32210, RRID:AB_621842 |
(1:10000) |
| Antibody | IRDye 800CW (secondary) |
Li-Cor | Cat # 926–32211, RRID:AB_621843 |
(1:10000) |
| Sequence-based reagent |
HSP60 forward primer, 5'- GCAGAGTTCCTCAGAAGTTGG-3' |
DOI: 10.1186/ s12974-016-0486-x |
qRT-PCR | |
| Sequence- based reagent |
HSP60 reverse primer, 5'- GCATCCAGTAAGGCAGTTCTC-3' |
DOI: 10.1186/ s12974-016-0486-x |
qRT-PCR | |
| Sequence-based reagent |
HSPA1A (HSP70)
forward primer, 5'- GGAGGCGGAGAAGTACA-3' |
DOI: 10.1021/ cb500062n |
qRT-PCR | |
| Sequence-based reagent |
HSPA1A (HSP70)
reverse primer, 5'- GCTGATGATG GGGTTAACA-3' |
DOI: 10.1021/cb500062n | qRT-PCR | |
| Sequence-based reagent |
hsp-16.1/.11 forward primer, 5'- ACCACTATTTC CGTCCAGCT-3' |
DOI: 10.7554/ eLife.08833 |
qRT-PCR | |
| Sequence-based reagent |
hsp-16.1/.11 reverse primer, 5'- TGACGTTCCATCTGAGCCAT-3' |
DOI: 10.7554/ eLife.08833 |
qRT-PCR | |
| Sequence-based reagent |
hsp-16.2 forward primer, 5'- TCGATTGAAGCGCCAAAGAA-3' |
DOI: 10.7554/eLife.08833 | qRT-PCR | |
| Sequence-based reagent |
hsp-16.2 reverse primer, 5'- TCTCTTCGACGATTGCCTGT-3' |
DOI: 10.7554/ eLife.08833 |
qRT-PCR | |
| Sequence-based reagent |
hsp-16.41 forward primer, 5'- TCTTGGACGAACTCACTGGA-3' |
DOI: 10.7554/eLife.08833 | qRT-PCR | |
| Sequence- based reagent |
hsp-16.41 reverse primer, 5'- AGAGACATCGAGTTGAACCGA-3' |
DOI: 10.7554/eLife.08833 | qRT-PCR | |
| Sequence-based reagent |
hsp-16.48/.49
forward primer, 5'- CTCATGCTCCGTTCTCCATT-3' |
DOI: 10.7554/ eLife.08833 |
qRT-PCR | |
| Sequence-based reagent |
hsp-16.48/.49 reverse primer, 5'- GAGTTGTGATCAGCATTTCTCCA-3' |
DOI: 10.7554/ eLife.08833 |
qRT-PCR | |
| Sequence-based reagent | GFP forward primer, 5'- GGTCCTTCTTGAGTTTGTAAC-3' |
DOI: 10.1074/ jbc.C100556200 |
qRT-PCR | |
| Sequence- based reagent |
GFP reverse primer, 5'- CTCCACTGACA GAAAATTTG-3' |
DOI: 10.1074/jbc.C100556200 | qRT-PCR | |
| Sequence-based reagent |
SDHA forward primer, 5'- TGGTGCTGGTTGTCTCATTA-3' |
DOI: 10.1134/ S0003683813090032 |
qRT-PCR | |
| Sequence- based reagent |
SDHA reverse primer, 5'- ACCTTTCGCCTTGACTGTT-3' |
DOI: 10.1134/ S0003683813090032 |
qRT-PCR | |
| Sequence- based reagent |
HSPC3 forward primer, 5'- ATGGAAGAGA GCAAGGCAAA-3' |
DOI: 10.1134/ S0003683813090032 |
qRT-PCR | |
| Sequence-based reagent |
HSPC3 reverse primer, 5'- AATGCAGCAAG GTGAAGACA-3' |
DOI: 10.1134/ S0003683813090032 |
qRT-PCR | |
| Sequence- based reagent |
crn-3 forward primer, 5'- GAATGCACTCAT GAACAAAGTC-3' |
DOI: 10.7554/ eLife.08833 |
qRT-PCR | |
| Sequence- based reagent |
crn-3 reverse primer, 5'- TAATGTTCGACT GATGAACCG-3' |
DOI: 10.7554/ eLife.08833 |
qRT-PCR | |
| Sequence- based reagent |
xpg-1 forward primer, 5'- ATTGAGAACAG GATCATGAGG-3' |
DOI: 10.7554/eLife.08833 | qRT-PCR | |
| Sequence- based reagent |
xpg-1 reverse primer, 5'-ACTAGCA ACTCGTTTATCATCC-3' |
DOI: 10.7554/ eLife.08833 |
qRT-PCR | |
| Sequence-based reagent | rpl-6 forward primer, 5'-ACTAGCAACTCGTTTATCATCC-3' | DOI: 10.7554/eLife.08833 | qRT-PCR | |
| Sequence-based reagent |
rpl-6 reverse primer, 5'- GACAGTCTTGGAATGTCCGA-3' |
DOI: 10.7554/eLife.08833 | qRT-PCR | |
| Commercial assay or kit |
20S Proteasome Activity Assay Kit | Chemicon International | Cat # APT280 | |
| Commercial assay or kit | RNeasy Mini Kit | Qiagen | Cat # 74104 | |
| Commercial assay or kit | iScript RT-Supermix | Bio-Rad | Cat # 170–8841 | |
| Commercial assay or kit | SsoAdvanced SYBR Green Supermix | Bio-Rad | Cat # 172–5264 | |
| Commercial assay or kit | PROTEOSTAT Prot. aggregation assay | Enzo | Cat # ENZ-51023 | |
| Chemical compound, drug | minocycline | Mp Biomedicals | Cat # 0215571891 | |
| Chemical compound, drug | methyl viologen hydrate (paraquat) | Acros Organics | Cat # 227320010 | |
| Chemical compound, drug | tunicamycin | LKT Laboratories | Cat # T8153 | |
| Chemical compound, drug | thapsigargin | Cayman Chemical | Cat # 10522 | |
| Chemical compound, drug | ISRIB | R and D Systems | Cat # 5284/10 | |
| Chemical compound, drug | levamisole | Mp Biomedicals | Cat # 0215522810 | |
| Chemical compound, drug | sodium arsenite | Spectrum Chemical |
Cat # S1135 | |
| Chemical compound, drug | cycloheximide | Alfa Aesar | Cat # J66901-03 | |
| Software, algorithm |
CellProfiler | Broad Institute | RRID:SCR_007358 | α-synuclein::YFP aggregate number and worm size analyses |
| Software, algorithm |
Bio-Rad CFX Manager |
Bio-Rad | qRT-PCR analysis | |
| Software, algorithm |
ImageQuant | GE Healthcare Life Sciences |
RRID:SCR_014246 | 35S incorporation analysis |