Table I.
Primer sequences.
| Gene | Forward (5′-3′) | Reverse (5′-3′) |
|---|---|---|
| DLL4 | AGGCCTGGCACTCACCTTACTC | CACCCCAGCCCTCTTTACAGTT |
| NOTCH1A | GCCGCAGATGCAGGGCAATGAAGT | GAGGGCAGGCAGGGCTGGTAGAGG |
| HEY2 | CGGCTTCCGGGAGTGTCTGACT | TCCCCACGGTCGGTATGGTTTA |
| EFNB2A | TTGGGGCCTGGAGTTCTTCAGA | TCTTGGGCGTGGCTAATGTGCT |
| SLIT2 | GAGCGACTGGACCTGAATG | GTAGATCCTGAAATGCCCCTC |
| SLIT3 | GCGAGTGTTTCCAAGACCTG | GATTTCATTGTCGTTCAGCCG |
| ROBO1 | AGGAGTCACATACAGGCTAGAG | GTCTGAGATCTGCTGGGAAATG |
| ROBO2 | GAGGTGTGGATGTGGACTATG | CTACAATCCGAGGTGGAGAATC |
| ROBO4 | GAGATCAGTCCCAAACCACAG | CCCACAGATATAGCCCAACG |
| FGFR2 | CCCCGACAACCGCACGCTCGTA | TAGCCGCCCATGCGATCCTCCTGT |
| FGFR3 | TCCCCGTATCCAGGTATCC | TCTGAACGTGGGTCTTTGTG |
| COX2 | CCTTCCGGCCATCATTCTTATT | CCGCAGATTTCAGAGCATTGTC |
| PTP-RB | TTGGGCAGCATGCGGAATACTGAG | TTACCAGGCTGCCATGAAACATCC |
| VEGFAA | CTCCATCTGTCTGCTGTAAAGG | GGGATACTCCTGGATGATGTCTA |
| VEGFR-2 | CCTGAGACCATCTTTGACCG | GTTCCCTCTTTAAGTCGCCTG |
| VEGFR-1 | GTATTTGAACAGCACGGGTTTAG | CGGCTTCTTGATATGCGTTTG |
| PIK3R2 | CCCGGAAACTGCTCCCCCTAATCT | AGCGGGAGGAGTCGGCTCTTGTT |
| β-actin | TCCGGTATGTGCAAAGCCGG | CCACATCTGCTGGAAGGTGG |
FGFR, fibroblast growth factor receptor; SLIT3, slit guidance ligand 3; ROBO, roundabout guidance receptor; PTP-RB, protein tyrosine phosphatase, receptor type B; DLL, deltalike ligand 4; COX, cyclooxygenase; VEGFR, vascular endothelial growth factor receptor; PIK3R2, phosphoinositide-3-kinase regulatory subunit 2; HEY2, hes-related family basic helix-loop-helix transcription factor with YRPW motif 2; EFNB2, ephrin B2.