Key resources table.
| Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|
| HEK293 | DSMZ | ACC 305 | |
| HEK 293 stable stably expressing TRPML3-YFP |
Grimm et al. (2010); PMID: 20189104 |
||
| HEK 293 stable stably expressing TRPML1-YFP |
Chen et al. (2014), PMID: 25119295 |
||
| TRPML1 (encoded by the Mcoln1 gene) KO mouse; Mcoln1tm1Sasl, C57BL/6 |
Venugopal et al. (2007); PMID: 17924347 |
MGI ID: 3794204 | |
| TRPML2 (encoded by the Mcoln2 gene) KO mouse; C57BL/6 |
Sun et al. (2015); PMID: 26432893 |
MGI: 1915529 | |
| TRPML3 (encoded by the Mcoln3 gene) KO mouse; Mcoln3tm1. 1Hels, FVB/NJ |
Jörs et al. (2010); PMID: 21179200 |
MGI ID: 5319089 | |
| anti-LAMP-1 (1D4B) (rat monoclonal) |
Santa Cruz | Cat#A-11006; RRID: AB_2134495 | (1:100) |
| Goat anti-Rat IgG (H + L) Secondary Antibody, Alexa Fluor 488 |
ThermoFisher | Cat#sc-19992; RRID: AB_2534074 | (1:1000) |
| mcherry-Transferrin Receptor 20 (plasmid) |
N/A | Addgene Plasmid #55144 | |
| DsRed-Rab11 (plasmid) |
Choudhury et al. (2002); PMID: 12070301 |
Addgene Plasmid #12679 | |
| TRPML1-YFP (plasmid) |
Grimm et al. (2010), PMID: 20189104 |
||
| TRPML2-YFP (plasmid) |
Grimm et al. (2010), PMID: 20189104 |
||
| TRPML3-YFP (plasmid) |
Grimm et al. (2010), PMID: 20189104 |
||
| Quikchange primers for TRPML2:YFP A422C |
this paper | forward: CTTCGGTTTTGTTGTTGTG CTGGTATGATTTATCTGGG reverse: CCCAGATAAATCATACCAGC ACAACAACAAAACCGAAG |
|
| Quikchange primers for TRPML2:YFP A424V |
this paper | forward: CGGTTTTGTGCTTG TGTTGGTATGATTTATCTGGGTTACAC reverse: GTGTAACCCAGATAAATCAT ACCAACACAAGCACAAAACCG |
|
| Quikchange primers for TRPML2:YFP G425A |
this paper | forward: CGGTTTTGTGCTTGT GCTGCTATGATTTATCTGGGTTACAC reverse: GTGTAACCCAGATAAATCA TAGCAGCACAAGCACAAAACCG |
|
| Quikchange primers for TRPML2:YFP A453S |
this paper | forward: CTGAACACAGTTTCTG AGTGTCTGTTTTCTCTGG reverse: CCAGAGAAAACAGACA CTCAGAAACTGTGTTCAG |
|
| Quikchange primers for TRPML2:YFP V460I |
this paper | forward: TGTCTGTTTTCTCTGATCA ACGGTGATGACATG reverse: CATGTCATCACCGTTGATC AGAGAAAACAGACA |
|
| Quikchange primers for TRPML2:YFP I498V |
this paper | forward: CCTTCATCAGCCTTTTTATATATA TGGTTCTCAGTCTTTTTATTGC reverse: GCAATAAAAAGACTGAGAACCA TATATATAAAAAGGCTGATGAAGG |
|
| qPCR Primer for TRPML1 (NM_053177) |
www.pga.mgh.harvard.edu/primerbank | PrimerBankID: 16716462 c2 | forward: GCCTTGGGCCAATGGATCA reverse: CCCTTGGATCAATGTCAAAGGTA |
| qPCR Primer for TRPML2 (NM_026656) |
this paper | forward: AATTTGGGGTCACGTCATGC reverse: AGAATCGAGAGACGCCATCG |
|
| qPCR Primer for TRPML3 (NM_134160) |
this paper | forward: GAGTTACCTGGTGTGGCTGT reverse: TGCTGGTAGTGCTTAATTGTTTCG |
|
| qPCR Primer for HPRT (NM_013556) |
Hruz et al. (2011); PMID: 21418615 |
N/A | forward: GCTCGAGATGTCATGAAGGAGAT reverse: AAAGAACTTATAGCCCCCCTTGA |
| Lipopolysaccharides (LPS) from Escherichia coli O26:B6 |
Sigma-Aldrich | Cat#L2762 | |
| Lipopolysaccharides (LPS) from Escherichia coli O111:B4 |
Sigma-Aldrich | Cat#L4391 | |
| Fura-2, AM, cell permeant |
ThermoFisher | Cat#F1201 | |
| Mouse M-CSF, premium grade |
Miltenyi Biotech | Cat#130-101-703 | |
| Transferrin from human serum, Alexa FluorTM 546-conjugated |
TermoFisher | Cat# T23364 | |
| Transferrin from human serum, Alexa FluorTM 555-conjugated |
Thermo Fisher | Cat#T35352 | |
| JE/MCP-1/CCL2 from mouse, recombinant |
Sigma-Aldrich | Cat# SRP4207 | |
| YM201636 | Chemdea | Cat#CD0181 | |
| MLSA-1 | Sigma-Aldrich | Cat#SML0627 | |
| PI(3,5)P2 | AG Scientific | Cat#P-1123 | |
| Wortmannin | Sigma-Aldrich | Cat#W1628 | |
| LatrunculinB | Sigma-Aldrich | Cat#L5288 | |
| Vacuolin | Santa Cruz | Cat# sc-216045 | |
| Calcium ionophore A23187 | Sigma-Aldrich | Cat#C7522 | |
| 4-Methylumbelliferyl N-acetyl-b-D-glucosaminide |
Sigma-Aldrich | Cat#M2133 | |
| RNeasy Plus Mini Kit | Qiagen | Cat# 74134 | |
| RevertAid first strand cDNA synthesis Kit |
ThermoScientific | Cat# K1621 | |
| CD11b MicroBeads, human and mouse |
Miltenyi Biotech | Cat#130-049-601 | |
| QuikChange II Site-Directed Mutagenesis Kit |
Agilent | Cat#200523 | |
| Mouse/rat CCL2/ JE/MCP-1 Quantikine ELISA Kit |
BioLegend | Cat#432707 | |
| Origin8 | OriginLab | ||
| GraphPad Prism | GraphPad Software Inc. |