Figure 2.

miR-34a directly targets Notch1. (A) Interaction between miR-34a and 3′UTR of Notch1 was predicted by TargetScan. (B) Luciferase activity of a reporter containing a Notch1 3′UTR-WT or 3′UTR-MUT (with a mutation in the miR-34a binding site). (C) Western blots and (D) quantified expression of Notch1 protein in MCF-7 cells in different groups, expressed as the fold of the NC group. (E) Relative mRNA expression level of Notch1 in MCF-7 cells in different groups. *P<0.05 and **P<0.01 vs. NC group. miR, microRNA; NC, negative control; UTR, untranslated region; WT, wild-type; MUT, mutated.