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. 2018 Nov 5;43(1):345–357. doi: 10.3892/ijmm.2018.3975

Figure 5.

Figure 5

miR-214 binds to MEG3 and ATF4. Primary hepatocytes were co-transfected with pcDNA3.1-MEG or control pcDNA3.1 vectors, and miR-214 mimic or NC sequences. (A) RT-qPCR and (B) western blot analysis results indicated that simultaneous overexpression of miR-214 and MEG3 significantly decreased the MEG3-mediated upregulation of ATF4 mRNA and protein expression. β-actin served as the loading control in the western blot analysis. (C) Results of the luciferase reporter assay demonstrated that the miR-214 mimic led to a marked decrease in luciferase activity in the MEG3-Wt reporter group compared with the miR-NC group. There was no marked effect on luciferase activity in the MEG3-Mut reporter group. (D) Luciferase activity in 293 cells co-transfected with Wt ATF4 3′UTR luciferase reporter plasmids and miR-214 mimics was decreased compared with that in the miR-NC group. *P<0.05 vs. the control + NC group; #P<0.05 vs. the NC+MEG3 group; &P<0.05 vs. the control group. MEG3, maternally expressed gene 3; ATF4, activating transcription factor 4; miR, microRNA; NC, negative control; Wt, wild-type; Mut, mutant.