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. 2018 Oct 19;43(1):83–90. doi: 10.3892/ijmm.2018.3944

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Copyright: © He et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Stability and structure integrity of exosome. (A) SDS-PAGE protein pattern. Colloidal Coomassie-stained gel of exosome fractions isolated by UC and OUF. In total, 20-µl urinary exosomes were loaded per lane in the order of morning, middle and night. Sizes of reference bands are indicated on the left in kilodaltons (kDa). (B) RT-qPCR detection of miR-205 and miR-7-5p levels in urinary exosomes from health donor samples that were collected at three different time and respectively isolated by UC and OUF. Results are expressed as the mean ± standard deviation (n=3; 3 biological replicates, with 3 technical replicates each). ^*P<0.05. UC, ultracentrifugation; OUF, optimized ultrafiltration.