Table I.
Primer sequences for reverse transcription-quantitative polymerase chain reaction.
| Gene name | Protein encoded | Forward primer (5′-3′) | Reverse primer (5′-3′) | Amplicon size (bp) |
|---|---|---|---|---|
| ACTA2 | α-SMA | ACTGCCTTGGTGTGTGACAA | CACCATCACCCCCTGATGTC | 120 |
| CNN1 | Calponin | AGGTTAAGAACAAGCTGGCCC | GAGGCCGTCCATGAAGTTGT | 113 |
| TAGLN | SM22α | CACAAGGTGTGTGTAAGGGTG | GGCTCATGCCATAGGAAGGAC | 132 |
| CCN2 | CCN2 | GTGCCTGCCATTACAACTGTC | TCTCACTCTCTGGCTTCATGC | 98 |
| ED-A Fn | ED-A Fn | CAGTGGAGTATGTGGTTAGTGTC | GTGACCTGAGTGAACTTCAGG | 119 |
| Col1A1 | Col1A1 | GTCAGGCTGGTGTGATGGG | GCCTTGTTCACCTCTCTCGC | 182 |
| Col1A2 | Col1A2 | CTGGAGAGGCTGGTACTGCT | AGCACCAAGAAGACCCTGAG | 62 |
| Col3A1 | Col3A1 | GGACACAGAGGCTTCGATGG | CTCGAGCACCGTCATTACCC | 190 |
| MMP1 | MMP1 | GCATATCGATGCTGCTCTTTC | GATAACCTGGATCCATAGATCGTT | 110 |
| LOX | LOX | CTCTTGCTGTCCTCCGCTC | ATCTTGGTCGGCTGGGTAAG | 155 |
| MYOCD | MYOCD | AGAGGCCATAAAAGGTAACCAGG | GGGGGTCTTCACTTCGAGTC | 116 |
| TGFB1 | TGF-β1 | CATTGGTGATGAAATCCTGGT | TGACACTCACCACATTGTTTTTC | 110 |
| TGFB2 | TGF-β2 | GAGCGACGAAGAGTACTACG | TTGTAACAACTGGGCAGACA | 89 |
| TGFBR1 | TGF-βR1 | GCAGACTTAGGACTGGCAGTAAG | AGAACTTCAGGGGCCATGT | 104 |
| TGFBR2 | TGF-βR2 | TGGACCCTACTCTGTCTGTG | CTGGAGCCATGTATCTTGCAG | 72 |
| TGFBR3 | Betaglycan | CTGGTGTGGCATCTGAAGAC | GGACCACAGAACCCTCAGAC | 79 |
| GAPDH | GAPDH | GAGTCCACTGGCGTCTTCAC | TTCACACCCATGACGAACAT | 119 |
ACTA2, α-SMA; α-SMA, smooth muscle α actin; CNN1, calponin 1; TAGLN, transgelin; CCN2, connective tissue growth factor; CTGF, CCN2; ED-A Fn, ED-A fibronectin; Col1A1, collagen type 1 α 1; MMP1, matrix metallopeptidase 1; LOX, lysyl oxidase; MYOCD, myocardin; TGF-β1, transforming growth factor-β1; TGFB1, TGF-β1; TGF-βR1, TGF-β receptor 1; TGFBR1, TGF-βR1.