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. 2018 Oct 23;43(1):103–116. doi: 10.3892/ijmm.2018.3950

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Copyright: © Jiang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Establishment of mouse hepatic stellate cell lines stably expressing Galectin-1 at low or high levels. (A) RT-qPCR results show that the mRNA expression level of Galectin-1 was higher in the overexpressed Galectin-1 groups. (B) A western blot assay and (C) quantification of results confirmed that the protein expression level of Galectin-1 was higher in the overexpressed Galectin-1 groups; ^*P<0.05 vs. control group. (D) RT-qPCR results that the mRNA expression level of Galectin-1 was highest for shRNA1 among the shRNA groups. (E) A western blot assay and (F) quantification confirmed that the protein expression level of Galectin-1 was highest for shRNA1 among the shRNA groups;^*P<0.05 vs. sh-NC group; ^#P<0.05 vs. shRNA1 group; ^&P<0.05 vs. shRNA2 group. sh, short hairpin RNA; NC, negative control; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.