Figure 1.

Schmetic of ESCO2 expression that is increased in CRC.

Notes: (A) Transcriptome sequencing (RNA-Seq) using eight paired fresh CRC and NT tissues and another four CRC specimens was performed. The expression of ESCO2 is indicated (left panel), and the alteration of ESCO2 expression in paired samples is presented (right panel). Horizontal lines: mean expression level. (B) The increase in ESCO2 mRNA was validated in 211 pairs of clinical specimens using qRT-PCR. (C) ESCO2 upregulation was also found in other studies from oncomine data set. (D) ESCO2 protein expression was determined in 16 paired CRC tissues. Representative Western blot results (left panel) and the related expression levels (right panel) are presented. (E) Expression of ESCO2 protein examined in 106 paraffin-embedded tissues. Representative IHC images (left panel) and scores (right panel) are indicated. (F) Expression of ESCO2 in CRC tissues was compared in patients with or without lymph node metastasis, lymphovascular invasion, distal metastasis, and vascular invasion.

Abbreviations: CRC, colorectal cancer; IHC, immunohistochemistry; NT, nontumorous; qRT-PCR, quantitative real-time PCR; TCGA, The Cancer Genome Atlas; FC, fold change; RSEM, RNA-seq by expectation-maximization.