Figure 4.

Effects of MOK extract on production of pro-inflammatory cytokines induced by LPS in RAW 264.7 cells. Cells were incubated with MOK extract for 6 h with or without LPS. (A) RT-PCR was used to measure the mRNA level of each cytokine (TNF-α, IL-1β and IL-6) with GAPDH as an internal control (ratios are in relation to GAPDH). (B) Data represent means ± standard error of the mean of three independent experiments. ^*P<0.05 and ^**P<0.01 vs. 1st bar, untreated control cells (a) or 2nd bar, cells treated with LPS only (b). Mean densitometric values of the three independent experiments were analyzed and are expressed as bar charts. LPS, lipopolysaccharide; RT-PCR, reverse transcription-polymerase chain reaction; TNF, tumor necrosis factor; IL, interleukin; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.