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. 2018 Oct 16;43(1):26–36. doi: 10.3892/ijmm.2018.3937

Figure 5.

Figure 5

Effects of MOK extract on phosphorylation of MAPKs induced by LPS in RAW 264.7 cells. After treatment with the indicated MOK extract concentrations for 30 min, cells were stimulated for 5 min with LPS (for ERK) or for 15 min with LPS (for JNK and p38 MAPK). (A) The cellular proteins were used to detect the phosphorylated or total forms of the MAPK molecules ERK1/2, JNK and p38 MAPK with β-actin as the housekeeping control protein. (B) Data represent means ± standard error of the mean of three independent experiments. *P<0.05, **P<0.01 and ***P<0.001 vs. 1st bar, untreated control cells (a) or 2nd bar, cells treated with LPS only (b). Mean densitometric values of the three independent experiments were analyzed and are expressed as bar charts. MAPK, mitogen-activated protein kinase; LPS, lipopolysaccharide; ERK, extracellular signal-regulated kinase; JNK, c-Jun N-terminal kinase.