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Crystallization conditions for BfL complexes.
| Ligand | Composition of the precipitant solution |
|---|---|
| L305 Tn-168(o)* Tn-207 |
25% (w/v) PEG1500, 0.1 M Bis-Tris Propane pH 9.0, 0.1 M NaCl |
| Gb4 | 28% (w/v) PEG6000, 0.1 M Tris pH 8.5, 0.5 M lithium chloride |
| Tn-168(m)* | 11% (w/v) PEG8000, 0.1 M Hepes pH 7.5, 8% (v/v) ethylene glycol |
| Tn-200 | 24% (w/v) PEG1500, 0.1 M Hepes pH 7.5, 0.2 M L-proline |
Letters ‘o’ and ‘m’, in parenthesis, refer to the orthorhombic and monoclinic crystals, respectively.
