Figure 4:
CD4 T cell subsets express different patterns of integrin expression: CD4+, CD45RA-antigen-experienced T cells, gated by FACS from the blood lymphocyte singlets of 25 IBD patients (described in supplemental table 2), were stained for surface receptors associated with Th1 (CXCR3+), Th2 (CRTh2+, CCR4+), Th17 (CD161+, CCR6+) and cTFH (CXCR5+) subsets, representative gating strategy of which is shown in (A). (B) The percent of each subset expressing both α4 and β7, just α4 (presumably with integrin β 1), or neither integrin were compared by paired Friedman test. (C) The percent of antigen-experienced (CD45RA-) CD4+ T cells resembling Th1, Th2, Th17 or cTFH cells by receptor expression were compared between Crohn’s disease (CD) and either healthy (Ctrl) or multiple sclerosis (MS) cohorts by Kruskal-Wallis H test. If the latter was significant (p<0.05), additional two-way testing was performed between the CD cohort and each of the other two cohorts by Mann-Whitney U testing. Asterisks under p-values denote their significance after adjusting for multiple comparisons. “ns” denotes non- significance.