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. Author manuscript; available in PMC: 2018 Nov 27.
Published in final edited form as: HardwareX. 2018 Feb 12;3:49–61. doi: 10.1016/j.ohx.2018.02.001

Fig. 1.

Fig. 1

PrintrBot Simple Metal modified with the LVE for FRESH printing. The build plate of the printer holds a Petri dish of FRESH support bath into which the needle is lowered to perform printing. The Bowden tubing, comprised of polyurethane and acquired online from Grainger (See Bill of Materials), separates the 60 mL disposable syringe (here containing Alcian-Blue-dyed alginate) from the needle, allowing the 3D printed LVE core to be mounted to the frame of the PrintrBot as shown. The LVE leadscrew is also driven by the stock NEMA 17 stepper motor that came with the PrintrBot. The stepper motor has simply been removed from the stock hot end assembly and screwed into the LVE assembly as shown; no other alterations to the motor are required for this set up.