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. Author manuscript; available in PMC: 2018 Nov 27.
Published in final edited form as: Biochem Biophys Res Commun. 2014 May 14;449(2):248–255. doi: 10.1016/j.bbrc.2014.05.018

Figure 2. Tcf3|Ascl1, Tcf4|Ascl1, and Tcf12|Ascl1 heterodimers binding modified CGs in two 8-mer: CGCAG|GTG and CGCAA|ATG.

Figure 2

A) EMSA showing a half-log dilution from 30 nM to 0.3 nM for the Tcf3|Ascl1 heterodimer binding two dsDNA 28-mers containing CGCAG|GTG, unmodified and containing 5caC in the CG dinucleotide. The right panel shows 3 nM Tcf3|Ascl1 heterodimer binding to 25 DNAs (see Figure 1). (B) EMSA showing a dilution from 1,000 nM to 10 nM for the Tcf3|Ascl1 heterodimer binding two dsDNA 28-mers containing a weak E-Box (CGCAA|ATG). The right panel shows binding of 300 nM Tcf3|Ascl1 heterodimer to 25 DNAs. (C) EMSA showing a dilution from 200 nM to 2 nM for the Tcf12|Ascl1 heterodimer binding two dsDNAs 28-mers containing CGCAG|GTG. The right panel shows 60 nM Tcf12|Ascl1 heterodimer binding to 25 dsDNAs. D) Tcf4|Ascl1.