Table 3.
The CD thermal denaturation monitored at 245 nm for four dsDNA 28-mers containing CGCAG|GTG (a) where the two cytosines in the CG dinucleotide are either C or 5caC. Thermal denaturation of Tcf3|Ascl1 heterodimer and Ascl1 homodimer monitored at 222 nm bound to four DNAs.
CGCAG|GTG (°C) | |||
---|---|---|---|
DNA (E-Box) | DNA (°C±S.E.) |
DNA+Tcf3|Ascl1 (°C±S.E.) |
DNA+ Ascl1 (°C±S.E.) |
C(a)|C(b) (○) | 74.2±0.27 | 61.4±0.15 | 43.6±0.65 |
C(a)|5caC(b) (◑) | 68.3±0.27 | 61.8±0.18 | 41.2±0.51 |
5caC(a)|C(b) (◐) | 68.9±0.30 | 64.4±0.18 | 48.5±0.38 |
5caC(a)|5caC(b) (●) | 67.4±0.28 | 64.2±0.07 | 47.8±0.15 |