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. 2018 Nov 13;2018:6816508. doi: 10.1155/2018/6816508

Figure 1.

Figure 1

β3-AR affects the glycolytic metabolism of both A375 melanospheres and embryonic stem cell. WB analysis of β3-AR, hexokinase II (HKII), monocarboxylate transporter-4 (MCT-4), and GLUT-1 markers (a). Analysis of the lactate export and glucose uptake (b). ATP production assay (c). A375 melanospheres and mouse ES cells treated with BRL37344 (10 μM) and BRL37344 (10 μM) + SR59230A (10 μM). The following experiments were performed: representative random figure of melanospheres in each condition and clonogenesis assay quantification (d); analysis of CD133+ cells by FACS and WB of stem cell marker SOX-2 (e); glucose uptake and lactate export in A375 melanospheres and ES cells (f, g); WB analysis of HKII and MCT-4 in A375 melanospheres (h) and ES cells (i). Data are representative of at least three experiments (mean and SD). ∗∗ P < 0.01 and ∗∗∗ P < 0.001.