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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Mol Biochem Parasitol. 2018 Nov 3;226:20–23. doi: 10.1016/j.molbiopara.2018.10.003

Fig. 1.

Fig. 1.

A diagram of the ES promoter reporter constructs. The difference between the two cassettes is only the identity of the promoter, MES (from the T. brucei Lister 427 VSG397 ES) or BES (from the T. brucei Lister 427 VSG2 ES). Different nucleotides between the two promoters are underlined and the position immediately upstream of the Pol I transcription start site is marked with −1. The backbone of the constructs is pBluescript II KS(+). AGs indicate the trans-splice sites upstream and downstream of the BSD coding sequence. The Mlu I restriction site present in the β/α-tubulin intergenic region was used to linearize the plasmids prior to transfection.