Table 2. Omissions in published papers by authors of this article.

We also show where STRENDA DB would have avoided those omissions.

Issues found in papers	Where could STRENDA DB help?
Michele Cianci, Bartlomiej Tomaszewski, John R. Helliwell, and Peter J. Halling (2010) “Crystallographic Analysis of Counterion Effects on Subtilisin Enzymatic Action in Acetonitrile” J. Am. Chem. Soc. 132, 2293–2300. In Materials & Methods, under “Cs Soaking, Cross-Linking, and Acetonitrile Wash” there is “So the soak solution was prepared freshly by mixing 2 volumes of 25% aqueous glutaraldehyde with 3 volumes of 250 mM Na-cacodylate buffer pH 6.5 containing 25% v/v glycerol and, where required, 1.5 M salt. Crystals were soaked in this for 15 min, with vortexing in the case of suspensions of multiple crystals for activity assay. Washing in acetonitrile followed immediately.” The presentation of the buffer and pH does not make it clear whether:	STRENDA DB requires entry of the pH, which should be the final value in the assay mixture. Further details of the treatment of pH are a planned enhancement.
a) 250 mM Na-cacodylate pH 6.5 was first prepared, then 25% glycerol was added, or
b) pH 6.5 is an apparent pH reading in the aqueous-glycerol mixture from an electrode calibrated in dilute aqueous buffer, or
c) pH 6.5 refers to an absolute pH scale established for 25% aqueous glycerol, with a protocol using appropriate standards in this mixture.
Richard S. Hall, Rakhi Agarwal, Daniel Hitchcock, J. Michael Sauder,Stephen K. Burley, Subramanyam Swaminathan and Frank M. Raushel (2010) “Discovery and Structure Determination of the Orphan Enzyme Isoxanthopterin Deaminase” Biochemistry 49, 4374–4382. Under “Measurement of Enzymatic Activity”, there is “The coupling system contained 7.4mM alpha-ketoglutarate, 0.4mM NADH, 6 units of glutamate dehydrogenase, and 100 mM HEPES (pH8.5) in a final volume of 0.25mL.” The cation present in the HEPES buffer is not stated. Also, the range of substrate concentrations used to measure the kinetic constants was not clearly reported.	STRENDA DB requires entry of buffer counter-ions if help recommendations are followed. In addition, STRENDA DB requires entry of substrate concentrations, either a fixed value or a range.
Wolfgang E. Schäfer, Johann M. Rohwer and Frederik C. Botha (2004) “A kinetic study of sugarcane sucrose synthase”. Eur. J. Biochem. 271, 3971–3977.
In the description under “SuSy assays”, the assay temperature is not specified.	STRENDA DB requires the assay temperature.
Table 1 does not provide error estimates for Ki values.	STRENDA DB requires error estimates for all kinetic constants entered.