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. Author manuscript; available in PMC: 2019 Sep 26.
Published in final edited form as: ACS Appl Mater Interfaces. 2018 Sep 14;10(38):32782–32791. doi: 10.1021/acsami.8b07573

Figure 1.

Figure 1.

Membrane-less compartmentalization using ATP-PDDA coacervates in a 10% DEX – 10% PEG system. (A) Reconstructed Z-stack image of a rhodamine B-encapuslating ATP-PDDA droplet (red) resuspended in a DEX droplet (green) in the PEG phase (black). Red: rhodamine B and green: FITC-DEX. (B) Time-lapse release of ATP from the coacervate quantified by UV-VIS spectroscopy measurement in MES (closed triangle) or the DEX (closed circle) solutions over 24 hours. The released ATP was normalized by the total ATP in the coacervate (mol%). The total ATP was obtained by complete coacervate dissociation by NaCl addition. (C) A brightfield image of the ATP-PDDA coacervates encapsulating food colorings (blue, red, yellow, and green) in the DEX-PEG ATPS. Scale bar (A) 500 μm and (C) 1 mm.