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A colorimetric assay of the cascade reaction under compartmentalized and released conditions. (A) The compartmentalized HRP-DAB in the absence of 200 mM NaCl at t = 90 min. (B) The released HRP-DAB in the presence of 200 mM NaCl at t = 90 min. (C) Absorbance change of DAB (l = 471 nm) and oPD (l = 492 nm) using compartmentalized or released HRP-DAB or HRP-oPD. Scale bar 1 mm. The bar indicates individual pairwise differences: * p < 0.05 and *** p < 0.01.
