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. Author manuscript; available in PMC: 2018 Nov 27.
Published in final edited form as: J Am Chem Soc. 2018 Jun 12;140(24):7381–7384. doi: 10.1021/jacs.8b02176

Figure 3.

Figure 3.

In vitro detection of protein aggregates. (a) Protein aggregates provide a crowded environment to restrict rotational motion of 3 and turn on its fluorescence. (b) Aggregation kinetics of α-synuclein as measured by 3 and ThT. (c) Heat-induced aggregation of SOD1(V31A) increases fluorescence intensity of 3. (d) Kinetics of fluorescence increase is faster than that of turbidity increase during aggregation of SOD1(V31A) at 54.5 °C.