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. Author manuscript; available in PMC: 2018 Nov 27.
Published in final edited form as: J Am Chem Soc. 2018 Jun 12;140(24):7381–7384. doi: 10.1021/jacs.8b02176

Figure 4.

Figure 4.

FP chromophore analogue 4 enables a fluorogenic method (AggTag) to detect aggregates of specific proteins in live cells. (a) Diagram of the fluorogenic AggTag method that detects POI aggregation in live cells. (b) Structure of 4. (c) Aggregation of the Htt 97Q-Halo·4 conjugate induces fluorescence increase. (d) Fluorescent image of cell lysate from cells expressing Q0-Halo or Q97-Halo labeled by 4 after fractionation. Quantification of fluorescent intensity is shown in Figure S13c. T, total lysate; S, supernatant; I, insoluble fraction. Methods are provided in SI Method Sections 4 and 13.