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AggTag method detects stress-induced protein aggregates that are invisible using nonfluorogenic methods. (a) Fluorogenic detection of NaAsO2-induced SOD1(V31A) aggregation in HEK293T cells. (b) NaAsO2 treatment leads to more SOD1(V31A) aggregation as confirmed by fractionation. Methods are provided in SI Method Sections 4, 5, and 13.
