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. Author manuscript; available in PMC: 2019 Apr 8.
Published in final edited form as: Nat Microbiol. 2018 Oct 8;3(12):1354–1361. doi: 10.1038/s41564-018-0256-x

Figure 4. Vpx counteracts FAM208A restriction of exogenous HIV-1 and SIVMAC239 infection and of endogenous LINE-1.

Figure 4.

a,b, Replication of HIV-1-ZsGreen in Jurkat cells transduced with SIVMAC251 vpx or control (a), or with lentivectors expressing shRNA targeting FAM208A or Luc control (b). Replication kinetics was measured by flow cytometry for ZsGreen+ cells. c,d, Spreading infection of HIV-1-ZsGreen (c), SIVMAC239 or SIVMAC239Δvpx (d) in CEMx174 cells transduced with FAM208A or Luc control shRNA. Spread of HIV-1-ZsGreen was assessed by flow cytometry as in (a), while spread of SIVmac239 (d) was assessed by measuring the accumulation of reverse transcriptase (RT) activity in the supernatant. All data is representative of at least four repeat experiments. Data from biological replicates was normalized to maximum infection observed in that experiment and the area under the curve was calculated for statistical comparison (see Supplementary Fig. 4). e, FAM208A, LINE-1 ORF1p, and Actin immunoblot, of CEMx174 and NTERA-2D1 cells, transduced with SIVMAC251 vpx or control, or lentivectors expressing shRNA targeting FAM208A or Luc control (n=3).