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. 2018 Nov 26;10:148. doi: 10.1186/s13148-018-0582-0

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Validation of GF-specific gene expression and its correlation with active and repressive histone marks. a IGV genome browser view of RNA-seq and ATAC-seq signals on the indicated loci from abdominal (red) and GF (green) preadipocytes. Overlay tracks are presented for each gene loci. b ChIP-qPCR assay in abdominal and GF preadipocytes with H3K4me3 antibody and H3K27me3 antibody at the promoter region of the selected genes. c RT-qPCR assay for HOXC13 and HOTAIR in abdominal and GF preadipocytes. Wilcoxon test *p < 0.05. White squares represent the IgG negative control