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Primer sequences for RT-PCR
| mRNA targets | Primer sequence (5’−3’) | |
|---|---|---|
| TNF-α | F |
CATGAGCACAGAAAGCATGATC |
| R | CCTTCTCCAGCTGGAAGACT | |
| IL-6 | F | ATGGATGCTACCAACTGGAT |
| R | TGAAGGACTCTGGCTTTGTCT | |
| IL-1β | F | CAACCAACAAGTGATATTCTCCATG |
| R | GATCCACACTCTCCAGCTGCA | |
| IL-2 | F | TGAGCAGGATGGAGAATTACAGG |
| R | GTCCAAGTTCATCTTCTAGGCAC | |
