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. 2018 Nov 26;37:284. doi: 10.1186/s13046-018-0956-3

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — MNAT1 interacts with p53. A, the lysates of LoVo cells were immunoprecipitated using anti-IgG, anti-MNAT1 or anti-p53 antibodies, and immunoprecipitation products were analyzed by Western-blotting with the indicated antibody. B, HEK293T cells were cotransfected with Flag-MNAT1 and HA-p53. Flag or HA was immunoprecipitated in the transfected cells, and the immunocomplexes were detected using Western-blotting with HA- or Flag-antibody. C, The various construct plasmids of TP53 were constructed using PCR (a). HEK293T cells were cotransfected with Flag-MNAT1 and the indicated plasmids of TP53. Immunoprecipitation was performed using anti-HA antibody, and the immunoprecipitated products were detected using Western-blotting with anti-Flag antibody. D, The plasmids containing various MNAT1 gene segments were constructed (a). HEK293T cells were cotransfected with HA-TP53 and the indicated plasmids of Flag-MNAT1. Immunoprecipitation was performed using anti-Flag antibody, and the immunoprecipitated products were detected using Western-blotting with anti-HA antibody. FL, full length p53; IP, immunoprecipitation; WCL, whole cell lysate