Table 3.
Effect of weaning stress on the relative gene expression of CXCL8, IL-1β, GRα, TLR4, TNFα
| Variable | Days post weaning1 | P-values3 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Group | 0 | 1 | 2 | 3 | 7 | 14 | 21 | SEM | T2 | S | T × S | |
| CXCL8 | C | 1.65 | 3.16c | 3.27c | 4.34c,x | 4.11c,x | 4.13c,x | 3.52c,x | 0.15 | ** | *** | *** |
| H | 2.46 | 2.69 | 2.97 | 2.18y | 2.09y | 2.41y | 2.34y | 0.12 | ||||
| IL-1β | C | 1.89 | 2.09x | 1.77x | 3.22c,x | 3.17b,x | 4.22c | 5.40c,x | 0.20 | ** | *** | *** |
| H | 2.68 | 3.37y | 4.11c,y | 5.37c,y | 4.70c,y | 4.77c | 4.04c,y | 0.16 | ||||
| GRα | C | 1.53 | 2.72c | 4.92c | 5.94c,x | 6.97c,x | 6.70c,x | 3.63c | 0.28 | *** | *** | *** |
| H | 1.06 | 2.07b | 4.50c | 4.30c,y | 3.17c,y | 3.57c,y | 3.58c | 0.18 | ||||
| TLR4 | C | 2.55 | 2.36x | 2.94x | 3.02x | 3.63b | 4.43c,x | 3.84b | 0.14 | *** | *** | *** |
| H | 3.00 | 4.04b,y | 5.50c,y | 4.48c,y | 3.06 | 2.60y | 3.12 | 0.16 | ||||
| TNFα | C | 2.63 | 3.80c | 7.29c,x | 7.96c,x | 6.74c,x | 2.79x | 2.90 | 0.32 | *** | *** | *** |
| H | 2.42 | 3.22 | 3.69b,y | 2.96y | 2.57y | 3.73b,y | 2.39 | 0.13 | ||||
Values are expressed as least squares means (LS-means)
11, 2, 3, 7, 14, and 21 d relative to weaning (= 0 d)
2T: MR treatment; S: sampling time; T × S: MR treatment × sampling time interaction
3Superscripts a, b, c within rows to indicate the LS-means differ from 1, 2, 3, 7, 14, and 21 d compared with 0 d by P < 0.05, P < 0.01, and P < 0.001, respectively; x, y within columns, to indicate that the LS-means differ from C and H groups by P < 0.05; * P < 0.05, ** P < 0.01, *** P < 0.001