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. 2018 Nov 12;12(11):e0006939. doi: 10.1371/journal.pntd.0006939

Fig 4. Multilocus conventional PCR for T. cruzi genotyping with the reference strains/clones.

Fig 4

PCR product sizes are indicated on the left by arrows. In A, SL-IR I and II target: Lanes—1: negative control; 2: Dm28c clone (TcI); 3: Y strain (TcII); 4: INPA 4167 strain (TcIV); 5: no template control (NTC). In B, 24Sα (second round) target: Lanes—1: NTC; 2: negative control; 3: Y strain; 4: INPA 3663 strain (TcIII); 5: Bug 2149 clone (TcV); 6: CL strain (TcVI). In C, A-10 target: 1: NTC, 2: Y strain (TcII), 3: CL strain (TcVI), 4: negative control. M- DNA ladder (100 or 50 bp).