Fig. 5.

Gene structures and AS events. a Distribution of transcripts that have one or more exons from Iso-Seq. b Distribution of transcripts that have one or more exons from reference genome. c The total number of AS events in detected genes by SMRT compared with the annotated gene models. Annotation, AS events in genes detected by SMRT based on reference genome; Iso-seq, AS events in genes detected by SMRT based on Iso-Seq reads. SE, skipped exon; MX, mutually exclusive exon; A5, alternative 5′ splice site; A3, alternative 3′ splice site; RI, retained intron; AF, alternative first exon; AL, alternative last exon. d Distribution of genes that produce one or more splice isoforms from Iso-Seq and annotation gene models