Fig. 5.

Overexpression USP37 elevates cell self-renewal and chemo-resistance to cisplatin. a Expressions of USP37, Smo, Gli-1, ALDH1 and OCT4 were detected in MCF-7-CTL or MCF-7-USP37 cells by western blotting. *P < 0.05, **P < 0.01. b Comparison of mammosphere formation among MCF-7-CTL or MCF-7-USP37 cells. *P < 0.05. c MCF-7-CTL and MCF-7-USP37 cells were treated with cisplatin (1 μg/ml) for 48 h, then treated cells were harvested in order to detect the levels of USP37, Bcl-2 and Bax. GAPDH was examined as a loading control. *P < 0.05. d CCK-8 assay showed that cell viability was enhanced in MCF-7 after USP37 upregulation compared to the control group. *P < 0.05, **P < 0.01, ***P < 0.01