Fig. 7.

USP37 interacts with and stabilizes Gli-1 protein. a, b MCF-7 and MDA-MB-231 cells were transfected with shScramble and shUSP37#2, respectively, then treated with 10 μM of MG132 4 h before harvesting. c Immunofluorescence staining of Gli-1 after USP37 knockdown or USP37 overexpression (Scale bar: 50 μm). d, e Cells transfected with shScramble and shUSP37#2 were treated with 50 μg/ml of CHX and harvested at the indicated time point. f MCF-7-CTL and MCF-7-USP37 cells were incubated with 50 μg/ml of CHX and harvested at the indicated time point. Gli-1 expression was analyzed by western blotting and quantified with Image-Pro Plus 6.0 software. g Exogenous USP37 and Gli-1 magnified strong, positive overlapping expression in MCF-7 cells by immunofluorescent staining (Scale bar: 50 μm). h CO-IP assay showed the interaction between endogenous USP37 and Gli-1 in MCF-7 cells