Fig. 8.

USP37 knockdown inhibits tumorigenicity and increases sensitivity to cisplatin in vivo. a, b, c MCF-7 cells transfected with shScramble or shUSP37#2 were subcutaneously injected into BALB/c nude mice treated with 0.9% saline or cisplatin. Tumors were excised from nude mice at day 28. a Images of tumor xenografts and b tumor weight for tumor xenografts treated with 0.9% saline or cisplatin are shown above. *P < 0.05, **P < 0.01. c Tumor growth curves were drawn every other day up to day 25. d Western blotting analysis of USP37, Smoothened, Gli-1, ALDH1, OCT4 and Bcl-2 in tumor xenografts. GAPDH was used as a loading control. *P < 0.05, **P < 0.01. e HE staining and immunohistochemical detection of USP37, Smoothened, Gli-1, Ki-67 in tumor xenografts. Scale bar: 100 μm