Table 1. Information on primers, probes and genes for real-time PCR.

Target gene	Primer sequences	Accession number
GAPDH1	Forward: 5’-GTCGGAGTGAACGGATTTGG-3’ Reverse: 5’-CAATGTCCACTTTGCCAGAGTTAA-3’ Probe: 5’-CGCCTGGTCACCAGGGCTGCT-3’	AF017079
TNF-α2	Forward: 5’-AACCCTCTGGCCCAAGGA-3’ Reverse: 5’-GGCGACGGGCTTATCTGA-3’ Probe: FAM-TCAGATCATCGTCTCAAAC-MGB	X57321
COX-23	Forward: 5’-GGGACGATGAACGGCTGTT-3’ Reverse: 5’-CACAATCTTAATCGTTTCTCCTATCAGT-3’ Probe: 5’-AGACGAGCAGGCTGA-3’	NM_214321
IL-104	Forward: 5’-GAGGAGGTGAAGAGTGCCTTTA-3’ Reverse: 5’-CTCACCCATGGCTTTGTAGACA-3’ Probe: FAM-CCTCTCTTGGAGCTTGC-MGB	L20001
ZO-15	TaqMan Gene Expression Assay. catalogue no. 4351370. assay ID ss03373514_m1 (Applied Biosystems. Thermo Fisher Scientific)
OCLN6	TaqMan Gene Expression Assay. catalogue no. 4351370. assay ID ss03377507_u1 (Applied Biosystems. Thermo Fisher Scientific)
CLDN-27	TaqMan Gene Expression Assay. catalogue no. 4351370. assay ID ss03375002_u1 (Applied Biosystems. Thermo Fisher Scientific)
CLDN-48	TaqMan Gene Expression Assay. catalogue no. 4351370. assay ID ss03375006_u1 (Applied Biosystems. Thermo Fisher Scientific)

¹GAPDH = glyderaldehyde-3-phosphate dehydrogenase;

²TNF-α = tumour necrosis factor-α;

³COX-2 = cyclooxygenase-2;

⁴IL-10 = interleukin-10;

⁵ZO-1 = zonula occludens-1;

⁶OCLN = occludin;

⁷CLDN-2 = claudin-2;

⁸CLDN-4 = claudin-4