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. 2018 Nov 27;13(11):e0207915. doi: 10.1371/journal.pone.0207915

Fig 1. Acetylation status of the chromatin and α-tubulin in lung fibroblasts of patients with idiopathic pulmonary fibrosis in response to treatment with LBH589 or pirfenidone.

Fig 1

Primary IPF-fibroblasts (n = 4) were incubated for 24h with vehicle [Veh., 0.25% (v/v) DMSO], panobinostat (LBH589, 85 nM) or pirfenidone (Pirf., 2.7 mM), followed by harvesting of cells. Acetylation status of histone H3 (A, B) and α-tubulin (C, D) was analyzed by quantitative immunoblotting. In dependency of research target, histone H3 or GAPDH served as loading control. (A) Histone H3-acetyl K27 (antibody-dilution 1:15000), (B) Histone H3-acetyl K27 (antibody-dilution 1:2000), (C) acetylated α-tubulin (antibody-dilution 1:60000), (D) acetylated α-tubulin (antibody-dilution 1:2000). Data are presented as median ± range of the individual values of different treatments. *p<0.05, by Mann Whitney test.