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. 2018 Nov 27;9:5021. doi: 10.1038/s41467-018-07448-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Medial and junctional Myosin-II distinctly tunes Vinc/E-cad ratio distribution. Representative images showing the distribution of Myosin-II (a) and Vinc/E-cad ratio (b), in the water injection “control” embryos (left) and H1152-injected “Rok inhibition” embryos (right). c, d Quantifications showing the levels of Myosin-II in medial and junction pool, along with planar polarity. Data from n = 7 embryos for both controls and Rok inhibitions. e Quantifications showing the mean junctional Vinc/E-cad ratio, along with planar polarity. Data from n = 6 embryos for controls and n = 7 for Rok inhibitions. Representative images showing the distribution of Myosin-II (f) and Vinc/E-cad ratio (g), in the control embryos (left) and RhoGEF2-RNAi embryos (right). h, i Quantifications showing the levels of Myosin-II in medial and junctional pool, along with planar polarity. Data from n = 8 embryos for both RhoGEF2-RNAi and control. j Quantifications showing the mean junctional Vinc/E-cad ratio along with planar polarity. Data from n = 6 embryos for control and n = 7 embryos for RhoGEF2-RNAi. Representative images showing the distribution of Myosin-II (k) and Vinc/E-cad ratio (l), in the control embryos (left) and Gα12/13 over-expressing embryos (right). m, n Quantifications showing the levels of Myosin-II in medial and junctional pool along with planar polarity. Data from n = 7 embryos for both Gα12/13 over-expression and control. o Quantifications showing the mean junctional Vinc/E-cad ratio along with planar polarity. Data from n = 7 embryos for control and n = 6 embryos for Gα12/13 over-expression. All scale bars represent 5 μm. All error bars represent SEM. Statistical significance estimated using two-tailed Student’s t-test. Images/quantifications in a, c, d, f, h, i, k, and m, n come from embryo co-expressing MyoII-mCherry and E-cadherin-GFP, while those in b, e, g, j, l, and o come from embryo co-expressing Vinculin-mCherry and E-cadherin-GFP. The proportion of tagged vs untagged protein pool varies across different experiments (see Methods: Fly lines and genetics). In all cases, Junctions marked based on E-cadherin localization. Images/quantifications in b and e are from the same set of embryos as those presented in Figure 1o–q. ns, p > 0.05; *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001