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. 2018 Nov 27;9:5021. doi: 10.1038/s41467-018-07448-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Medial Myosin-II tunes the levels of junctional E-cadherin. a Representative images showing the distribution of E-cadherin, in the genetic outcross control embryos (left) and RhoGEF2-RNAi embryos (right). b Quantifications showing the reduction in E-cadherin levels, quantified as mean junctional intensity. Data from n = 8 embryos for both RhoGEF2-RNAi and control. c Representative images showing the distribution of E-cadherin, in the genetic outcross control embryos (left) and Gα12/13 over-expressing embryos (right). d Quantifications showing an increase in E-cadherin levels, quantified as mean junctional intensity. Data from n = 7 embryos for both Gα12/13 over-expression and control. All scale bars represent 5 μm. All error bars represent SEM. Statistical significance estimated using two-tailed Student’s t-test. Images/quantifications in all panels come from embryo co-expressing MyoII-mCherry and E-cadherin-GFP, though the proportion of tagged vs untagged protein pool varies across different experiments (detailed in Methods: Fly lines and genetics). Corresponding Myosin-II related images/quantifications are presented in Figure 4. In all cases, Junctions marked based on E-cadherin localization. ns, p > 0.05; *, p < 0.05; **, p < 0.01; ***, p < 0.001