Fig. 3. Effect of culture temperature on the persistence of the filamentous phenotype.

Filamentous cells were plated on Lee’s glucose and Lee’s GlcNAc media for 7 days of growth at 37, 25, or 20 °C. Cellular morphology of representative colonies was examined. Percentage of filamentous cells are indicated. Approximately 1500–2000 cells were examined for each culture. Scale bar represents 10 μm