Fig. 7. Virulence of typical yeast and filamentous C. auris cells in a mouse model of fungal burden.

a Fungal burdens were determined using excised leg muscle samples. Mice were injected with typical yeast or filamentous cells intraperitoneally. After 24 h of infection, mice were euthanized humanely. b Periodic acid–schiff (PAS) stained sections of spleens from infected animals were imaged. Tissues from intraperitoneally injected mice were fixed, sectioned, and stained for microscopy assays. c Morphological analysis of C. auris cells recovered from leg tissues described in panel (a). d Fungal burdens as determined through in vivo systemic infection mouse model. Typical yeast or filamentous cells were injected via the mouse tail vein. Fungal burdens of multiple organs were analyzed following 24 h of infection. e Morphological analysis of C. auris cells recovered from organ tissue samples described in panel (d). Recovered cells were plated and grown on YPD medium at 30 °C for 2 days and then transferred at 25 °C for 5 days of incubation. Scale bar represents 10 μm