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. 2018 Nov 27;9:5006. doi: 10.1038/s41467-018-07344-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Effects of PTENα on HSV-P10 infected cells. a Increased mitochondrial membrane potential in HSV-P10 infected cells 6 hpi. Seeded cells were infected with either HSVQ or HSV-P10 at MOI = 0.5 for 6 h, and mitochondrial membrane potential indicated by JC-1 red fluorescence was imaged using fluorescence microscopy. Scale bar = 100 µm. b ATP release from infected cells 24 hpi. Data shown are mean ATP concentration in conditioned media from infected cells 24 hpi ± S.D. Statistical analysis performed with a 2-tailed Student’s T-test. (n = 3/group, **p < 0.01, ***p < 0.001). c ATP measured in cell lysates of infected cells 24 hpi. Data shown are mean ATP concentration 24 hpi ± S.D. Statistical analysis performed with a 2-tailed Student’s T-test. (n = 3/group, **p < 0.01, ***p < 0.001). d Western blot of DB7 cells 24 hpi with HSVQ (Q), HSV-P10 (P), or left untreated (NV) ± PI3Ki (LY294002). L indicates the molecular weight ladder. e ATP release from DB7 or MDA-468 cells 12 hpi with HSVQ or HSV-P10. HSVQ and PI3Ki treated samples were also evaluated for ATP release. Data shown are mean ATP concentrations in conditioned media from infected cells ± SEM. Statistical analysis performed with a 2-tailed Student’s T-test (n = 6/group, *p < 0.05, ***p < 0.001, n.s. = not significant)