Fig. 2.

Whole-genome sequencing of wild-type Pb and PbVac parasites before and after cyclical propagation and clonal expansion. a Insertion site of the construct in the P230p locus of Pb’s chromosome 3. b The PfCS gene is covered by reads from the PbVac parasite lines, showing that it is contained in the genome of the genetically modified parasite. c Artemis screenshot showing the copy number variation of the PbUIS4 gene in the wild-type Pb and PbVac parasites (top) and the “not properly paired” reads of the transgenic parasite lines (bottom). For each panel, the coverage plot is represented at the top and the mapped reads are shown at the bottom. Black: wild-type Pb prior to cyclic propagation; gray: wild-type Pb after cyclic propagation and clonal expansion; blue: PbVac prior to cyclic propagation; red: PbVac after cyclic propagation and clonal expansion