Fig. 5.

Toxicology of and humoral responses elicited by PbVac administration to NZW rabbits. a Schedule of parasite administration, sample collection, and killing (arrows) in rabbits subjected to the bites of 100 non-infected (black horizontal line, 5 male and 5 female NZW rabbits for each experimental group) or 100 PbVac-infected mosquitoes (orange horizontal line 5 male and 5 female NZW rabbits for each experimental group). b, c “Vaccine take” illustrated by the amount of anti-PbCS repeats (b), anti-PfCS repeats (c) antibodies detected in serum samples of mock-immunized and PbVac-immunized male and female rabbits, collected throughout the duration of the study (bars represent average of all observations per time point and respective standard deviation). d Body weight of male and female rabbits throughout the duration of the study. e Body temperature of male and female rabbits throughout the duration of the study. d, e Light and dark blue represent male rabbits subjected to non-infected mosquito bites or PbVac-infected mosquito bites, respectively, and light and dark pink represent female rabbits subjected to non-infected or PbVac-infected mosquito bites, respectively. Dots represent the average value of all animals within the group and bars indicate the standard deviation