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OST suppress proliferation of NRK-49F cells. NRK-49F were preincubated with OST for 30 min before 10% FBS and were harvested 24 h after FBS stimulation. (A) MTT and (B,C) EdU incorporation assays of different groups (magnification of 200×). Scale bar = 100 μm. Representative bands (D) and western blot analyses (E) of PCNA, cyclin D1 and p21 cip1. Data are expressed as mean ± SEM. ∗P < 0.05 versus untreated cells; #P < 0.05 versus FBS-stimulated cells.
