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. 2018 Nov 27;9:5004. doi: 10.1038/s41467-018-07446-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Dxz4 is necessary but not sufficient for megadomain formation. a Schematic of prominent features in the Dxz4 region and deletions generated in this study and previous studies. b Top: Contact maps for the Xi at 2.5 Mb resolution for wild-type (left) and Dxz4^∆/∆ (right) cells on d10 of differentiation. Bottom: Pearson correlation matrices at 1 Mb resolution for the wild-type (left) and Dxz4^∆/∆ Xi (right). Heatmaps generated by merging reads together from two biological replicates. c 1st principal component of the 1 Mb correlation matrix plotted for wild-type (black) and Dxz4^∆/∆ (red) across all bins on the Xi. The dotted line corresponds to the bin containing Dxz4. d Strategy for using 4C to localize the transgene insertion site. e, f 4C interaction profiles using a viewpoint in the backbone of an Xist transgene in either wild-type (top), Xist only Tg (middle) or Xist + Dxz4 Tg (bottom) lines across chr14 (e) or chr10 (f). g Hi-C contact maps for chr14 at 1 Mb resolution in either wild-type (left), Xist only Tg (middle left), Xist + Dxz4 Tg Dox induced for 2 days (middle right) or 9 days (right). The position of the Xist + Dxz4 transgene insertion site at ~69.8 Mb (near Stc1) is indicated by a green bar in the Xist + Dxz4 Tg contact maps