miR-370-3p Also Targets FOXM1, but Not FOXO1 and TGFβ-RII
LN18 cells were untreated or treated with mimetic miR-370-3p or mutated mimetic miR-370-3p. qRT-PCR and ELISA analyzed the expression level of FOXM1 (A), FOXO1 (B), and TGFβ-RII (C) at mRNA and protein levels, respectively. Mimetic wild-type or mutated miR-370-3p (25 nM) were transfected using HiPerFect transfection reagent (QIAGEN, France) according to the manufacturer’s instructions. A t test (GraphPad software) compares the mean ± SD of indicated values. Histograms represent average ± SD of 3 independent experiments of the considered parameters.