Table 2.

Prognosis Value of miRNA-370-3p Targets

	Validation Methods			Prognosis Value
	Reporter Assay	Western Blot	qPCR	Log-Rank Test	Expression Associated with a Favorable Prognosis
TGFβ-RII	✔	✔	✔	p = 0.336	–
FOXO1	✔	✔	✔	p = 0.248	–
FOXM1	✔	✔	✔	p = 0.0219	low

Here, the targets for miRNA-370-3p considered to be identified by the miRTarBase website. We considered targets validated by three strong pieces of evidence, i.e., targets validated by the use of three different methods (reporter assay, western blot, qPCR) commonly used to analyze the effect of miRNA on its targets (according to miRTarBase website criteria). Prognosis values were calculated using the Betastasis website and the REMBRANDT (repository for molecular brain neoplasia data) bioinformatics knowledgebase available for GBM (http://www.betastasis.com/glioma/rembrandt/kaplan_meier_survival_curve).