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. 2017 May 4;8(5):e2766. doi: 10.1038/cddis.2017.165

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Knockdown of MDIG inhibits tumour cell growth in vitro and in vivo. (a, b) MDIG mRNA (a) and its protein (b) level were detected, respectively, by qRT-PCR and by western blot in Huh7 and MHCC-97H cells as a quality control after MDIG knockdown (shMDIG) with shRNA in vitro. MOCK and NC were, respectively, served as blank and negative control. (c) Silencing expression of MDIG (shMDIG) decreases the cell growth of HCC cells by MTT assay. (d) Silencing expression of MDIG (shMDIG-1, -2, -3) remarkably reduced the colony formation ability of HCC cells, and bar graphs showed their quantitative analysis data. (e) Liver and lung tissues from the animals with tumour xenografts inoculated with Huh7 cell line stably expressing shMDIG-3, NC as a negative control; and bar graphs showed their quantitative analysis of the liver weights. (f) MDIG protein level was detected by western blot in Huh7 cell xenografts of shMDIG. The graph of (a), (c), (d) and (e) are shown as mean±s.d. of n=3 independent experiments. Unpaired Student’s t-tests were used for statistical analysis (*P<0.05, **P<0.01, ***P<0.001)